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Advanced Courses in Life Sciences

1st Edition

Experimental Entomology: Measurement and Analysis of Physiological and Behavioural Variables

September 11th-15th, 2017, Barcelona (Spain)

Experimental Entomology: Measurement and Analysis of Physiological and Behavioural Variables

The overall objective of this course is that participants become familiar with:

  1. Laboratory and field methodologies necessary for conducting experiments with insects.
  2. Sampling methods commonly used for different taxa.
  3. Aspects to consider for breeding and maintenance of insects in the laboratory to carry out experiments.

To accomplish this, the course will consist of an introductory part on aspects of physiology, ecology and behaviour of insects required for the experimental analysis. Then we will address the understanding of the importance of the development of the environmental conditions of work, protocols and experimental designs, as well as the correct measurement of physiological and behavioural through homebuilt experimental devices. Finally, the most common laboratory experimental problems will be addressed and tips for your best solution will be provided.

LOCATION

Centre de Restauració i Interpretació Paleontològica

C/ Mestre J. Lladós, 1
08781 Els Hostalets de Pierola, Barcelona.

How to get there

LANGUAGE

English

COURSE LENGTH & ECTS

40 hours on-site.

This course is equivalent to 2 ECTS (European Credit Transfer System) at the Life Science Zurich Graduate School.

The recognition of ECTS by other institutions depends on each university or school.

PLACES

Places are limited to 20 participants and will be occupied by strict registration order.

Participants who have completed the course will receive a certificate at the end of it.

Instructors

Claudio R. Lazzari instructor for Transmitting Science

Dr. Claudio R. Lazzari
Université François Rabelais de Tours
France

Marcela K. Castelo instructor for Transmitting Science

Dr. Marcela K. Castelo
Universidad de Buenos Aires
Argentina

José Emilio Crespo instructor for Transmitting Science

Dr. José Emilio Crespo
Universidad de Buenos Aires
Argentina

Coordinators

Ana Rosa Gómez-Cano coordinator at Transmitting Science

Dr. Ana Rosa Gómez-Cano
Transmitting Science
Spain

Soledad De Esteban-Trivigno instructor at Transmitting Science

Dr. Soledad De Esteban-Trivigno
Transmitting Science
Spain

Requirements

Graduate or postgraduate degree in any Biosciences discipline. All participants must bring their own personal laptop (Windows).

Program

1. Insect physiology and behaviour: Some important concepts for experimental analysis. Body organization, main functions.

2. Experimenting with insects: Controlling working conditions and environmental factors, dealing with interindividual variation, anesthesia and pain.

3. Measuring physiological and behavioural variables: From simple observation to the automatic registration. Quantitative analysis, organizing data.

4. Insect sensorial ecology: From the periphery to the central nervous system. Mechanoreception, olfaction, thermoreception, spatial color visión and polarization. Acquiring and using information, Communication, behavioural modulation of responses, state dependency.

5. Chemical Ecology: Substances that mediate interactions between insects, extraction, identification and analysis of biological function. The detectability-confiability dilema of information and its solutions. Experimental design and protocol development.

6. Adaptation to extreme conditions, thermal stress, thermoregulation and reparative mechanisms. Physiological mechanisms and adaptive processes. Global warming and its influence on insects life.

7. Experimental design and data treatment. Pseudoreplication in experimental biology: Problems and solutions.

8. Collecting methods and raising and maintenance in the laboratory.

9. bliometry and the evaluation of scientific production. Presenting results: Information organization in oral and written presentations.

10. Do it yourself: Practice with some tools and simple and useful devices for behavioural assays.

11. Practice measuring different behaviours with different experimental arenas. Orientation and preference assays, open and closed-loop conditions. Locomotion compensator.

12. Critical discussion of selected articles. Seminars of discussion.

Literature

Olfactive and visual modulation:

  • Vinauger C, Buratti & Lazzari CR, 2011a. Learning the way to blood: First evidence of dual olfactory conditioning in a blood-sucking insect, Rhodnius prolixus. I. Appetitive learning. The Journal of Experimental Biology, 214: 3032-3038.
  • Vinauger C, Buratti & Lazzari CR, 2011b. Learning the way to blood: First evidence of dual olfactory conditioning in a blood-sucking insect, Rhodnius prolixus. II. Aversive learning. The Journal of Experimental Biology, 214: 3039-3045.
  • Reisenman CE, Lazzari CR & Giurfa M, 1998. Circadian control of photonegative sensitivity in the haematophagous bug Triatoma infestans. Journal of Comparative Physiology A, 183: 533-541.  

Behaviour:

  • Barrozo RB & Lazzari CR. 2004. The response of the blood-sucking bug Triatoma infestans to carbon dioxide and other hosts odours. Chemical Senses, 29: 319-329.
  • Castelo MK & Lazzari CR. 2004. Host-seeking behavior in larvae of the robber fly Mallophora ruficauda (Diptera: Asilidae). Journal of Insect Physiology, 50(4): 331-336.
  • Franck NR & Richarson T. 2006. Teaching in tandem-running ants. Nature, 439 (12): 153.

Pseudoreplication:

  • Ramírez CC, Fuentes-Contreras E, Rodríguez LC & Niemeyer HM, 2000. Pseudoreplication and its frequency in olfactometric laboratory studies. Journal of Chemical Ecology, 26(6): 1423-1431.

 Physiology – Thermoregulation:

  • Lahondere C & Lazzari CR, 2012. Mosquitoes cool down during blood feeding to avoid overheating. Current Biology, 22: 40-45.
  • Seymour RS, White CR & Gibernau M, 2009. Endothermy of dynastine scarab beetles (Cyclocephala colasi) associated with pollination biology of a thermogenic arum lily (Philodendron solimoesense). The Journal of Experimental Biology, 212: 2960-2968.

Fees

  • Course Fee
  • Early bird (until February 28th, 2017):
  • 690
  • Regular (after February 28th, 2017):
  • 965
  • This includes course material, coffee breaks and lunches.
    (VAT included)
  • Accommodation Package
  • Early bird (until February 28th, 2017):
  • 360
  • Regular (after February 28th, 2017):
  • 360
  • This includes local transportation, lodging, breakfasts, dinners and social (tourist) activities.
    (VAT included)
  • Course Fee + Accommodation Package
  • Early bird (until February 28th, 2017):
  • 1,050
  • Regular (after February 28th, 2017):
  • 1,325
  • This includes course material, coffee breaks, local transportation, lodging, breakfasts, lunches, dinners and social (tourist) activities.
    (VAT included)

We offer the possibility of paying in two instalments (contact the course coordinators). Discounts (see Funding below) are not cumulative and apply only on the fee, not to Accommodation Package or other options.

Accommodation

If you take the Accommodation Package you will be hosted in the Youth Hostel Can Roviralta, in shared dormitories (common bathrooms), although you will only share rooms with other classmates. The possibility exists that the dormitories will be mixed. Therefore, if this is an inconvenience for you, and you require staying in a dormitory with only women, or only men, please indicate so in the “Comments” field of the Registration form (see below).

A supplemental charge will be added if you prefer to stay in a single room with private bathroom. The number of rooms is limited and if you prefer to have a single room, let us know it as soon as possible. If you want more information on this please contact the course coordinators.

Please be aware that from the centre of Barcelona city it takes about one hour by car and one hour and a half by public transport to arrive to Els Hostalets de Pierola. Course participants will find that staying in Els Hostalets de Pierola is more convenient.

If you do not want the Accommodation Package, accommodation is available in places other than Els Hostalets de Pierola. You may stay in Barcelona city, Martorell or Igualada. You will find information about Hotels in Martorell here, and information about Hotels in Igualada here.

How to get to Els Hostalets de Pierola from Barcelona city, Martorell and Igualada.

Youth Hostel Can Roviralta (Els Hostalets de Pierola, Barcelona, Spain)

Funding

Former participants will have a 5 % discount on the Course Fee.

Unfortunately there are no scholarships available for this course. However a 20 % discount on the Course Fee is offered for members of some organizations (Organizations with discount). If you want to apply to this discount please indicate it in the Registration form (proof will be asked later).

Spanish unemployed scientists, as well as Spanish PhD students without any grant or scholarship to develop their PhD, could benefit from a 40 % discount on the Course Fee. If you want to ask for this discount, please contact the course coordinators. That would apply for a maximum of 2 places and they will be covered by strict inscription order.

Discounts are not cumulative and apply only on the fee, not to Accommodation Package or other options.

Schedule

Course ScheduleCourse + Accommodation Package Schedule
Sunday, September 10th, 201719:00 Meeting point in Plaza Catalunya (Barcelona) to take the bus to Els Hostalets de Pierola. If you are planning to arrive later, you can find more information in How to get there.
20:00 Registration in the Youth Hostel.
20:30 Dinner.
Monday, September 11th through Friday, September 15th, 20179:30 to 13:30 Lessons.
13:30 to 15:00 Lunch.
15:00 to 19:00 Lessons.

There will be two coffee breaks each day, halfway through each lesson session.

8:30 to 9:00 Breakfast.
9:30 to 13:30 Lessons.
13:30 to 15:00 Lunch.
15:00 to 19:00 Lessons.
20:30 Dinner.

There will be two coffee breaks each day, halfway through each lesson session.

Saturday, September 16th, 20178:30 to 9:00 Breakfast.
9:15 Meeting point in the Youth Hostel to take the bus to visit a wine cellar.
10:00 Arrival at the wine cellar.
11:30 Bus to Barcelona city.
13:00 Arrival at Plaza Catalunya (Barcelona).

The schedule is approximate; it is possible that the content of one day may run into the next and a working day may be longer than advertised.

Organizers

Transmitting Science Logo
Centre de Restauració i Interpretació Paleontològica (CRIP) Logo
Institut Català de Paleontologia Miquel Crusafont (ICP) Logo

Collaborators

Colegio Oficial de Biólogos de Castilla y León Logo
Colegio Oficial de Biólogos de Euskadi Logo
Colexio Oficial de Biólogos de Galicia Logo
Col·legi Oficial de Biòlegs de la Comunitat Valenciana Logo
European Association of Vertebrate Palaeontologists (EAVP) Logo
Sociedade Brasileira de Paleontologia (SBP) Logo

Registration