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Advanced Courses in Life Sciences

2nd Edition

Introduction to Electron Microscopy for Life Sciences

July 4th-7th, 2017, Barcelona (Spain)


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Introduction to Electron Microscopy for Life Sciences

This course gives an introduction to both Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM). It covers electron microscopy principles and theory, as well as different fields of application, operation modes and image interpretation.

It is aimed at beginners and intermediate users of electron microscopes in life sciences. The course has a limited number of participants to allow small groups during practical sessions.

After this course, the participants should be able to align the microscopes, identify the most common aberrations and understand the principles of image generation in electron microscopy. Participants can also bring some samples already prepared on TEM grids or SEM stubs.


Campus de la Universitat Autònoma de Barcelona (UAB)

08193 Bellaterra (Cerdanyola del Vallès), Barcelona (Spain)




26 hours on-site (14.5 for practice and 11.5 for theory).

This course is equivalent to 1 ECTS (European Credit Transfer System) at the Life Science Zurich Graduate School and ForBio.

The recognition of ECTS by other institutions depends on each university or school.


Places are limited to 12 participants and will be occupied by strict registration order.

Participants who have completed the course will receive a certificate at the end of it.


Emma Rossinyol-Casals instructor for Transmitting Science

Dr. Emma Rossinyol-Casals
Universitat Autònoma de Barcelona

Alejandro Sánchez-Chardi instructor for Transmitting Science

Dr. Alejandro Sánchez-Chardi
Universitat Autònoma de Barcelona


Ana Rosa Gómez-Cano coordinator at Transmitting Science

Dr. Ana Rosa Gómez-Cano
Transmitting Science

Juan Vicente Bertó-Mengual coordinator at Transmitting Science

Juan Vicente Bertó-Mengual
Transmitting Science


Tuesday, July 4th, 2017.

Theory. Introduction to optics. Fundamentals of electron microscopy. Electromagnetic lenses. Vacuum systems. Electron guns. Scanning Electron Microscope (SEM). Columns and lenses. Image detectors. SEM principles.

Wednesday, July 5th, 2017.

Practical session. SEM and TEM basics I.

Theory. SEM. Energy Dispersive X-Ray Spectroscopy (EDS), Catodoluminiscence, Environmental SEM. Low voltage SEM, High Resolution SEM, non-conductive samples. Examples and applications. Sample preparation for SEM. Conventional method and immunolabeling. Critical point drying and surface coatings. Correlative microscopy.

Thursday, July 6th, 2017.

Practical session. SEM and TEM basics II.

Theory. Transmission Electron Microscope (TEM). Columns and lenses. Detectors. Electron Energy Loss Spectroscopy (EELS), Energy Filtered TEM (EFTEM) and High Angle Anular Dark Field (HAADF). Cryo TEM and Tomography. Sample preparation for TEM (conventional methods, cytochemistry, and cryomethods). Embedding and sectioning. Immunolabelling. Vitrification for cryo EM.

Friday, July 7th, 2017.

Practical session. SEM and TEM advanced I.

Practical session. SEM and TEM advanced II.

Several microscopes will be used: TEM JEOL 2011 (200KV), TEM JEOL 1400 (120KV), SEM ZEISS EVO, HRSEM ZEISS MERLIN. Practical sessions will be developed with small groups (6 people maximum).

Previous editions of this course


1st Edition, July 5th-8th, 2016

Course Introduction to Electron Microscopy for Life Sciences - 1st Edition, July 5th-8th, 2016Six people belonging to the following countries and institutions participated in this edition:

  • Sweden: Karolinska University Hospital.
  • The Netherlands: University of Amsterdam.
  • Colombia: Universidad Distrital Francisco José de Caldas.
  • United Kingdom: University of Cambridge.
  • Norway: Tromsø University Museum.
  • Denmark: Technical University of Denmark.


  • Course Fee
  • Early bird (until April 30th, 2017):
  • 730
  • Regular (after April 30th, 2017):
  • 1,020
  • This includes course material, coffee breaks and lunches.
    (VAT included)

We offer the possibility of paying in two instalments (contact the course coordinators).


Former participants will have a 5 % discount on the Course Fee.

Unfortunately there are no scholarships available for this course. However a 20 % discount on the Course Fee is offered for members of some organizations (Organizations with discount). If you want to apply to this discount please indicate it in the Registration form (proof will be asked later).

Discounts are not cumulative.


Course Schedule
Tuesday, July 4th through Friday 7th, 201710:00 to 14:00 Lesson.
14:00 to 15:30 Lunch.
15:30 to 18:00 Lessons.

There will be two coffee breaks each day, halfway through each lesson session.

The schedule is approximate; it is possible that the content of one day may run into the next and a working day may be longer than advertised.


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